9. Summary of darkfield observation procedures
Set the main swith to "ON" and adjust light intensity.
Place a slide on the stage.
Insert 10x objective into the light path
Focus the specimen
Lower the condenser, place a drop of oil on the front lens
and return the condenser to the original height
Rotate the nosepiece to an intermediate position between
the 40x and 100x objectives and place a drop of oil on the
Insert in the light path the 100x objective and focus the
Turn the iris diaphragm on the 100x objective to achieve the
Adjust interpupillary distance
Adjust diopter
Center optical axis
slide
specimen
Adjust brightness
darkfield effect
Adjust brightness
Begin observation
(Used commands)
Main switch /
brightness adjustment knob
Slide holder
X/Y movement knobs
Nosepiece
Coarse and fine focus knobs
Binocular tube
Diopter adjustment ring
Condenser height adjustment knob
Condenser centering screws
Condenser height adjustment knob
Nosepiece
Nosepiece
Coarse and fine focus knobs
Main switch /
brightness adjustment knob
100x OIL/IRIS objective
Main switch /
brightness adjustment knob
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