Adjustment - Thermo Fisher Scientific Thermo Scientific Finnpipette F1 Guide D'utilisation

Fixed Volume
µl
1
5
10
20
25
50
100
200
250
500
1000
2000
3000
5000
10000
Range Channel
1-10 µl 8, 12, 16
5-50 µl 8, 12, 16
10-100 µl 8, 12
30-300 µl 8, 12

Adjustment

Adjustment is done with the service tool.
1. Place the service tool into the openings of the
calibration nut at the top of the handle.
2. Turn the service tool clockwise to increase, or
counterclockwise to decrease the volume.
3. After adjustment check the calibration according
to the instructions above.
Formulas for calculating results
Conversion of mass to volume
V = (w + e) x Z
Evaporation loss can be significant with low volumes. To determine mass loss, dispense water
to the weighing vessel, note the reading and start a stopwatch. See how much the reading
decreases during 30 seconds (e.g. 6 mg = 0.2 mg/s).
Compare this to the pipetting time from taring to reading. Typically pipetting time might be 10
seconds and the mass loss is 2 mg (10 s x 0.2 mg/s) in this example. If an evaporation trap or lid
on the vessel is used the correction of evaporation is usually unnecessary.
The factor Z is for converting the weight of the water to volume at test temperature and pressure.
A typical value is 1.0032 µl/mg at 22°C and 95 kPa. See the conversion table on page 48.
Inaccuracy (systematic error)
Inaccuracy is the difference between the dispensed volume and the selected volume of a pipette.
A = V - V A =
0
V =
V =
0
Inaccuracy can be expressed as a relative value: A% = 100% x A / V
Inaccuracy
µl %
±0.050 ±5.00
±0.125 ±2.50
±0.120 ±1.20
±0.20 ±1.00
±0.50 ±2.00
±0.50 ±1.00
±0.80 ±0.80
±1.60 ±0.80
±4.00 ±1.60
±4.00 ±0.80
±8.00 ±0.80
±16.0 ±0.80
±40.0 ±1.33
±40.0 ±0.80
±60.0 ±0.60
Volume
µl
10
1
50
5
100
10
300
30
V = volume (µl)
w = weight (mg)
e = evaporation loss (mg)
Z = conversion factor for µl/mg conversion
inaccuracy
mean volume
nominal volume
8
Imprecision
s.d.µl
0.050
0.075
0.080
0.10
0.20
0.20
0.30
0.60
1.50
1.50
3.00
6.00
15.00
15.00
30.00
Inaccuracy
µl %
±0.24 ±2.4
±0.24 ±24
±1.0 ±2.0
±1.0 ±20
±0.80 ±0.8
±0.80 ±8.0
±8.0 ±2.7
±8.0 ±26.7
0
cv%
5.00
1.50
0.80
0.50
0.80
0.40
0.30
0.30
0.60
0.30
0.30
0.30
0.50
0.30
0.30
Imprecision
s.d.µl cv%
0.16 1.6
0.16 16
0.4 0.8
0.4 8.0
0.30 0.3
0.30 3.0
3.0 1.0
3.0 10.0
+