Thermo Fisher Scientific Thermo Scientific Finnpipette F1 Guide D'utilisation page 6

Forward technique
Fill a clean reagent reservoir with the liquid to be dispensed.
1 Depress the knob to the first stop.
2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm and slowly
release the knob. Withdraw the tip from the
liquid touching it against the edge of the reservoir
to remove excess liquid.
3. Deliver the liquid by gently depressing the knob
to the first stop. After a delay of about one
second, continue to depress the knob all the way
to the second stop. This action will empty the tip.
4. Release the knob to the ready position. If necessary, change the tip and continue pipetting.
Reverse technique
The reverse technique is suitable for dispensing liquids that have a high viscosity or a tendency
to foam easily. The technique is also recommended for dispensing very small volumes. Fill a clean
reagent reservoir with the liquid to be dispensed.
1. Depress the knob all the way to the second stop.
2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm, and slowly
release the knob. This action will fill the tip.
Withdraw the tip from the liquid touching it
against the edge of the reservoir to remove
excess liquid.
3. Deliver the preset volume by gently depressing
the knob to the first stop. Hold the knob at the first
stop. Some liquid will remain in the tip and this should not be included in the delivery.
4. The remaining liquid should either be discarded with the tip or pipetted back into
the container.
Repetitive technique
The repetitive technique offers a rapid and simple procedure for repeated delivery of the same
volume. Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the knob all the way to the second stop.
2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm, and slowly re
lease the knob. This action will fill the tip.
Withdraw the tipfrom the liquid touching against
the edge of the reservoir to remove excess liquid.
3. Deliver the preset volume by gently depressing
the knob to the first stop. Hold the knob at the
first stop. Some liquid will remain in the tip and
this should not be included in the delivery.
4. Continue pipetting by repeating steps 3 and 4.
Pipetting of heterogeneous samples
(deproteinization in blood glucose determination, for example)
Use steps 1 and 2 of the forward technique to fill the tip with blood.
Wipe the tip carefully with a dry clean tissue.
1. Immerse the tip into the reagent and depress the
knob to the first stop, making sure the tip is well
below the surface.
2. Release the knob slowly to the ready position.
This will fill the tip. Keep the tip in the solution.
3. Depress the knob to the first stop and release
slowly. Keep repeating this procedure until the
interior wall of the tip is clear.
4. Finally, depress the knob all the way to the
second stop to completely empty the tip.
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