Calibration And Adjustment - Thermo Fisher Scientific Thermo Scientific Finnpipette F2 Guide D'utilisation

Reverse technique
The reverse technique is suitable for dispensing liquids that have a high viscosity or a tendency
to foam easily. The technique is also recommended for dispensing very small volumes. Fill a
clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button all the way to the second stop.
2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm, and slowly
release the push button. This action will fill the
tip. Withdraw the tip from the liquid touching
it against the edge of the reservoir to remove
excess liquid.
3. Deliver the preset volume by gently depressing
the push button to the first stop. Hold the push
button at the first stop. Some liquid will remain
in the tip and this should not be included in
the delivery.
4. The remaining liquid should either be discarded with the tip or pipetted back into the
container.
Repetitive technique
The repetitive technique offers a rapid and simple procedure for repeated delivery of the same
volume. Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button all the way to the second stop.
2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm, and slowly re
lease the push button. This action will fill the tip.
Withdraw the tipfrom the liquid touching against
the edge of the reservoir to remove excess liquid.
3. Deliver the preset volume by gently depressing
the push button to the first stop. Hold the push
button at the first stop. Some liquid will remain
in the tip and this should not be included in
the delivery.
4. Continue pipetting by repeating steps 3 and 4.
Pipetting of heterogeneous samples
(deproteinization in blood glucose determination, for example)
Use steps 1 and 2 of the forward technique to fill the tip with blood.
Wipe the tip carefully with a dry clean tissue.
1. Immerse the tip into the reagent and depress the
push button to the first stop, making sure the tip
is well below the surface.
2. Release the push button slowly to the ready
position.
This will fill the tip. Keep the tip in the solution.
3. Depress the push button to the first stop and
release slowly. Keep repeating this procedure
until the interior wall of the tip is clear.
4. Finally, depress the push button all the way to the second stop to completely empty the tip.

Calibration and adjustment

All Finnpipettes are factory calibrated and adjusted to give the volumes as specified with distilled
or deionized water using the forward pipetting technique. It should be noted that the use of other
pipetting techniques may affect the calibration results. The pipettes are constructed to permit
re-adjustment for other pipetting techniques or liquids of different temperature and viscosity.
Device requirements and test conditions
An analytical balance must be used. The scale graduation value of the balance should be chosen
according to the selected test volume of the pipette:
1 2 3 4
1 2 3 4
1 2 3 4 5
6