Installing Stage Inserts; Aperture Diaphragm - Optika Italy IM Serie Manuel D'instructions

9.8.1 Installing stage inserts

1. Install the holder in the mechanical stage. (Fig. 40)
2. Multi well plates can be directly inserted in the mechan-
ical stage. (Fig. 41)
9.9

Aperture diaphragm

The Numerical Aperture (N.A.) value of the aperture
diaphragm affects the image contrast. Increasing or
reducing this value one can vary resolution, contrast and
depth of focus of the image.
With low contrast specimens move the Aperture Diaphragm
lever (AS) ① to set the numerical aperture to about 70%-
80% of the objective's N.A. (Fig. 42)
If necessary, remove one eyepiece and, looking into empty
sleeve, adjust the condenser's ring in order to obtain an
image like the one in fig. 43.
APERTURE
DIAPHRAGM
FIELD OF VIEW
Page 30
F F ig. 40 ig. 40
F ig. 41 F ig. 41
①
F ig. 42 F ig. 42
70-80%
30-20%
F ig. 43 F ig. 43