4. Use the CT to focus the condenser phase ring (bright)
① and the objective phase ring (dark) ② image. If the
bright phase ring's image is not sharp, adjust the CT
head until you can see a clear image of the phase ring.
(Fig. 48)
5. Adjust the bolts of the two centering holes in the phase
contrast slider using a screwdriver until the bright ring
and the dark ring match. (Fig. 49)
6. The 4x and the 10X phase contrast objectives use the
same ring on the phase contrast slider. The coincidence
of the phase ring center and the phase contrast center
must be verified with both objectives. (Fig. 50)
•
If the phase ring is incorrectly centered, the con-
trast will be severely impaired.
•
The phase ring may need recentering during and
after observation of very thick specimens.
•
The phase ring may show an apparent misalign-
ment if the specimen is not flat.
①
②
Page 33
F ig. 48
F
ig. 48
F ig. 49
F
ig. 49
F ig. 50
F
ig. 50