Use Of The Microscope In Phase Contrast (Transmitted Light); Installing The Phase Contrast Slider; Phase Contrast Slider; Centering The Phase Ring - Optika Italy IM Serie Manuel D'instructions

10. Use of the microscope in phase contrast (transmitted light)

10.1 Installing the phase contrast slider

1. Insert the slider into the illumination system, printed
face up. (Fig. 45)
2. Pull the slider into the desired position, until it arrives to
the click stop.
3. When in phase contrast observation, keep the aperture
diaphragm adjustment lever ① on the "O" (open) posi-
tion.

10.2 Phase contrast slider

• The phase ring is pre-centered when the microscope
leaves the factory. It should therefore need no further
adjustment. Should a re-centering is needed, it can be
performed via the two side bolts (see chapter 10.3).
• The 4x/10x position ② must be used with 4x and 10x
phase contrast objectives, the 20x/40x position ③ with
the 20x and 40x and the SL position ④ is used for
brightfield. (Fig. 46)
SLIDER
POSITION
SL
4x/10x phase ring 4x/10x
20x/40x phase ring 20x/40x

10.3 Centering the phase ring

• Usually this operation is not needed. If necessary,
please proceed with the following steps:
1. Place a specimen on the stage and focus it.
2. Take out the eyepiece from the eyepiece tube without
the diopter adjustment, and replace it with the centering
telescope (CT). (Fig. 47)
3. Check that the phase ring and the objective match, and
that both are steadily set on a click stop.
MEANING APPLICATION
brightfield
empty hole
observation
phase contrast
observation with
4x and 10x
objectives
phase contrast
observation with
20x and 40x ob-
jectives
Page 32
①
② ③
F ig. 45 F ig. 45
④
F ig. 46 F ig. 46
F ig. 47 F ig. 47