Digital Display; Pipette Operation; Pipetting Techniques - Thermo Fisher Scientific Thermo Scientific Finnpipette F2 Guide D'utilisation

Digital display

The adjusted delivery volume is clearly indicated in the large
digital display on the handle.
Raw materials
The Finnpipette F2 is made of mechanically durable and chemically resistant materials.
Description of tips
Finntips are recommended for use with the Finnpipette F2.
They are made of virgin natural colour polypropylene, generally regarded as the only
contamination free material suitable for tips. Finntips are also autoclavable (121°C).
Setting the delivery volume
1. Set the delivery volume using the push button on the top of
the pipette. To increase the delivery volume, turn the push
button counterclockwise. To decrease the delivery volume,
turn it clockwise.
2. Make sure that the desired delivery volume clicks into place.
3. Do not set volumes outside the pipette´s specified volume range.
Using excessive force to turn the push button outside the range
may jam the mechanism and eventually damage the pipette.
Safety Label
You can mark the pipette application, your initials, the
calibration date, etc. on the safety label. Remove the old
label with a sharp needle. Mark the new label with a pencil
and slide the label back in place.
Tip ejection
To help eliminate the risk of contamination, each pipette
is fitted with a tip ejector system. To release the tip, point
the pipette at suitable waste receptacle and press the tip ejector with your thumb.
Push and release the push button slowly at all times particularly when working with
high viscosity liquids. Never allow the push button to snap back.
Make sure that the tip is firmly attached to the tip cone. Check for foreign
particles in the tip.
Before you begin your actual pipetting work, fill and empty the tip 2-3 times with
the solution that you will be pipetting. Hold the pipette in an upright position while
aspirating liquid. The grippy should rest on your index finger. Make sure that the tips,
pipette and solution are at the same temperature.
Forward technique
Fill a clean reagent reservoir with the liquid to be dispensed.
1 Depress the push button to the first stop.
2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm and slowly
release the push button. Withdraw the tip from
the liquid touching it against the edge of the
reservoir to remove excess liquid.
3. Deliver the liquid by gently depressing the push
button to the first stop. After a delay of about
one second, continue to depress the push button
all the way to the second stop. This action will empty the tip.
4. Release the push button to the ready position. If necessary, change the tip and continue
pipetting.

Pipette operation

Pipetting techniques

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