Pipetting Techniques - Thermo Scientific Finnpipette Focus Multichannel Plus Guide D'utilisation

General
Special care shall be taken to avoid liquid being aspirated into the interior working of the
pipette.
Push and release the push button slowly at all times, particularly when working with high
viscosity liquids. Never allow the push button to snap back.
Make sure that the tip is firmly attached to the tip cone. Check for foreign particles in the tip.
Before you begin your actual pipetting work, fill and empty the tip 2-3 times with the
solution that you will be pipetting. Hold the pipette in an upright position while aspirating
liquid. The grippy should rest on your index finger. Make sure that the tips, pipette and
solution are at the same temperature.
Forward technique
Fill a clean reagent reservoir with the liquid to be
dispensed.
1. Depress the push button to the first stop.
2. Dip the tip under the surface of the liquid in
the reservoir to a depth of about 1 cm and
slowly release the push button.
Withdraw the tip from the liquid touching it
against the edge of the reservoir to remove
excess liquid.
3. Deliver the liquid by gently depressing the push button to the first stop. After a delay of
about one second, continue to depress the push button all the way to the second stop.
This action will empty the tip.
4. Release the push button to the ready position.
If necessary, change the tip and continue pipetting.
Reverse technique
The reverse technique is suitable for dispensing liquids that have a high viscosity or a
tendency to foam easily. The technique is also recommended for dispensing very small
volumes. Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button all the way to the second stop.
2. Dip the tip under the surface of the liquid in
the reservoir to a depth of about 1 cm and
slowly release the push button. This action
will fill the tip.
Withdraw the tip from the liquid touching it
against the edge of the reservoir to remove
excess liquid.
3. Deliver the preset volume by gently depressing
the push button to the first stop. Hold the
push button at the first stop. Some liquid will
remain in the tip and this should not be in
cluded in the delivery.
4. The remaining liquid should either be discarded with the tip or pipetted back into the container.
Repetitive technique
The repetitive technique offers a rapid and simple
procedure for repeated delivery of the same volume.
Fill a clean reagent reservoir with the liquid to be
dispensed.
1. Depress the push button all the way to the
second stop.
2. Dip the tip under the surface of the liquid in
the reservoir to a depth of about 1 cm and
slowly release the push button. This action will fill the tip. Withdraw the tip from the
liquid touching against the edge of the reservoir to remove excess liquid.

Pipetting techniques

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