Pipetting Techniques - Thermo Scientific Finnpipette F1 Guide D'utilisation

Push and release the knob slowly at all times particularly when working with high viscosity
liquids. Never allow the knob to snap back.
Make sure that the tip is fi rmly attached to the tip cone. Check for foreign particles in the tip.
Before you begin your actual pipetting work, fi ll and empty the tip 2-3 times with the solution
that you will be pipetting. Hold the pipette in an upright position while aspirating liquid. The
fi nger rest should rest on your index fi nger. Make sure that the tips, pipette and solution are at
the same temperature.
Forward technique
Fill a clean reagent reservoir with the liquid to be dispensed.
1 Depress the knob to the fi rst stop.
2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm and slowly
release the knob. Withdraw the tip from the
liquid touching it against the edge of the reservoir
to remove excess liquid.
3. Deliver the liquid by gently depressing the knob
to the fi rst stop. After a delay of about one
second, continue to depress the knob all the way
to the second stop. This action will empty the tip.
4. Release the knob to the ready position. If necessary, change the tip and continue pipetting.
Reverse technique
The reverse technique is suitable for dispensing liquids that have a high viscosity or a tendency
to foam easily. The technique is also recommended for dispensing very small volumes. Fill a
clean reagent reservoir with the liquid to be dispensed.
1. Depress the knob all the way to the second stop.
2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm, and slowly
release the knob. This action will fi ll the tip.
Withdraw the tip from the liquid touching it
against the edge of the reservoir to remove
excess liquid.
3. Deliver the preset volume by gently depressing
the knob to the fi rst stop. Hold the knob at the fi rst
stop. Some liquid will remain in the tip and this should not be included in the delivery.
4. The remaining liquid should either be discarded with the tip or pipetted back into
the container.
Repetitive technique
The repetitive technique offers a rapid and simple procedure for repeated delivery of the same
volume. Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the knob all the way to the second stop.
2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm, and slowly re
lease the knob. This action will fi ll the tip.
Withdraw the tipfrom the liquid touching against
the edge of the reservoir to remove excess liquid.
3. Deliver the preset volume by gently depressing
the knob to the fi rst stop. Hold the knob at the
fi rst stop. Some liquid will remain in the tip and
this should not be included in the delivery.
4. Continue pipetting by repeating steps 3 and 4.

Pipetting techniques

7
1 2 3 4
1 2 3 4
1 2 3 4