Calibration; Checking The Calibration - Thermo Scientific FINNPIPETTE COLOR Guide D'utilisation

ENGLISH
5. Pull out the piston.
6. Remove the O-ring from the piston.
7. Clean the piston, the piston spring
and the O-ring with a dry napless cloth.
8. Check the cylinder for foreign
particles.
9. Grease the cleaned parts with
lubricant provided in the package.
10. Reassemble the parts.
11. Check the calibration according to
instructions.

CALIBRATION

All Finnpipettes are factory calibrated
and adjusted to give the volumes as
specified with distilled or deionized
water. Normally, the pipettes do not
need adjustment, but they are
constructed to permit recalibration and
adjustment for liquids of different
temperature and viscosity.
DEVICE REQUIREMENTS AND
TEST CONDITIONS
An analytical balance must be used.
The scale graduation value of the
balance should be chosen according to
the selected test volume of the pipette:
Volume range
under 10 µl
10-100 µl
above 100 µl
Test liquid: Water, distilled or deionized,
"grade 3" water conforming ISO 3696.
Tests are done in a draft-free room at a
constant (±0.5°C) temperature of water,
pipette and air between 15°C to 30°C.
The relative humidity must be above
55%. Especially with volumes under
50µl the air humidity should be as high
as possible to reduce the effect of
evaporation loss. Special accessories,
such as the evaporation trap, are
recommended.

CHECKING THE CALIBRATION

The pipette is checked with the maximum
volume (nominal volume) and with the
minimum volume or 10% of maximum
volume, whichever is higher.
E.g. Finnpipette 0.5-10 µl is tested at
10 µ and 1 µ. A new tip is first pre-
wetted 3-5 times and a series of ten
pipettings are done with both volumes.
readable graduation
0.001 mg
0.01 mg
0.1 mg
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