Calibration - Thermo Scientific Finnpipette Guide D'utilisation Et D'entretien

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REVERSE TECHNIQUE
The reverse technique is suitable for dispensing liquids that have a high viscosity or a
tendency to foam easily. The technique is also recommended for dispensing very small
volumes.
Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button all the way to the second stop.
2. Dip the tip under the surface of the liquid in the reservoir to a depth of about 1 cm,
and slowly release the push button.
This action will fill the tip. Withdraw the tip from the liquid touching it against the
edge of the reservoir to remove excess liquid.
3. Deliver the preset volume by gently depressing the push button to the first stop.
Hold the push button at the first stop. Some liquid will remain in the tip and this
should not be included in the delivery.
4. The remaining liquid should either be discarded with the tip or pipetted back into the
container.
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REPETITIVE TECHNIQUE
The repetitive technique offers a rapid and simple procedure for repeated delivery of the
same volume. Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button all the way to the second stop.
2. Dip the tip under the surface of the liquid in the reservoir to a depth of about 1 cm,
and slowly release the push button. This action will fill the tip. Withdraw the tip from
the liquid touching against the edge of the reservoir to remove excess liquid.
3. Deliver the preset volume by gently depressing the push button to the first stop.
Hold the push button at the first stop. Some liquid will remain in the tip and this
should not be included in the delivery.
4. Continue pipetting by repeating steps 2 and 3.
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PIPETTING OF HETEROGENEOUS SAMPLES
(deproteinization in blood glucose determination, for example)
Use steps 1 and 2 of the forward technique to fill the tip with blood.
Wipe the tip carefully with a dry clean tissue.
1. Immerse the tip into the reagent and depress the push button to the first stop,
making sure the tip is well below the surface.
2. Release the push button slowly to the ready position. This will fill the tip. Keep the
tip in the solution.
3. Depress the push button to the first stop and release slowly. Keep repeating this
procedure until the interior wall of the tip is clear.
4. Finally, depress the push button all the way to the second stop to completely empty the tip.
All Finnpipettes are factory calibrated and adjusted to give the volumes as specified with
distilled or deionized water. The pipettes are constructed to permit re-adjustment for liquids
of different temperature and viscosity.

CALIBRATION

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