Thermo Scientific FINNPIPETTE COLOR Guide D'utilisation page 5

Wet the newly attached tip with the
solution being pipetted before any
actual pipetting takes place.
This is done by filling and emptying the
tip 2-3 times.
Hold the pipette vertically during the
liquid intake with the "Grippy" resting on
the index finger.
The temperature of the tip and pipette
should be equalized to that of the
solution.
FORWARD TECHNIQUE (Fig.4)
Fill a clean reagent reservoir with the
liquid to be dispensed.
1. Depress the operating button to the
first stop.
2. Dip the tip under the surface of the
liquid in the reservoir about 1cm deep
and slowly release the operating button.
Withdraw the tip from the liquid
touching it against the edge of the
reservoir to remove excess liquid.
3. Deliver the liquid by gently depressing
the operating button to the first stop.
After a delay of about one second,
continue to depress the operating
button all the way down to the second
stop. This action will empty the tip.
4. Release the operating button to the
ready position.
If necessary, change the tip and
continue with the pipetting.
REVERSE TECHNIQUE (Fig.5)
The reverse technique is suitable for
dispensing liquids having high viscosity
or a tendency to foam easily. The
technique is also recommended for
dispensing very small volumes.
Fill a clean reagent reservoir with the
liquid to be dispensed.
1. Depress the operating button all the
way down to the second stop.
2. Dip the tip under the surface of the
liquid in the reservoir about 1cm deep
and slowly release the operating button.
This action will fill the tip. Withdraw the
tip from the liquid touching it against the
edge of the reservoir to remove excess
liquid.
3. Deliver the preset volume by gently
depressing the operating button to the
first stop.
ENGLISH
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