Thermo Scientific FINNPIPETTE COLOR Guide D'utilisation page 9

A pipette is always adjusted for delivery
(Ex) of the selected volume. Measuring
volumes taken from balance is not
allowed. If the calculated results are in
the limits, the calibration of the pipette is
correct.
Procedure:
1. Do 10 pipettings with the minimum
volume.
2. Do 10 pipettings with the maximum
volume.
3. Calculate the inaccuracy (A) and
imprecision (cv) of both series.
4. Compare the results to the limits in the
Table 1.
If the results are in the limits of Table 1,
then the calibration of the pipette is
correct. Otherwise the pipette must be
adjusted and checked again.
Range
0,5-10µl
5-50µl
20-200µl
100-1000µl 1000
1-5 ml
ADJUSTMENT (Fig.10)
Adjustment is done with the service tool.
1. Place the service tool into the
openings of the calibration nut at the top
of the handle.
2. Turn the service tool clockwise to
increase, or counterclockwise to
decrease the volume.
3. After adjustment check the calibration
according to the instructions above.
FORMULAS FOR CALCULATING
RESULTS
Conversion of mass to volume
V = (w + e) x Z
V = volume (µl)
w = weight (mg)
e = evaporation loss (mg)
Z = conversion factor for mg/µl
conversion
Volume
µl
10
1
50
5
200
20
100
5000
1000
ENGLISH
Inaccuracy Imprecision
µl %
±0.10 ±1.0
±0.035 ±3.5
±0.30 ±0.6
±0.15 ±3.0
±1.2 ±0.6
±0.6 ±3.0
±5.0 ±0.5
±1.5 ±1.5
±25.0 ±0.5
±15.0 ±1.5
-9-
s.d.* µl cv%
0.08 0.8
0.03 3.0
0.15 0.3
0.13 2.5
0.6 0.3
0.3 1.5
2.0 0.2
0.6 0.6
10.0 0.2
5.0 0.5