STERILIZATION
The entire pipette can be sterilized by autoclaving it at 121°C (252°F) (minimum 20
minutes). No special preparations are needed for autoclaving. You can use steam
sterilization bags if needed.
After autoclaving the pipette must be cooled to room temperature for at least two hours.
Before pipetting, make sure that the pipette is dry. We recommend that you check the
calibration of after every 25th sterilization cycle.
The table below lists possible problems and their solutions.
Defect
Leakage
Inaccurate dispensing
Inaccurate dispensing
with certain liquids
TROUBLE SHOOTING
Possible reason
Tip incorrectly attached
Foreign particles
between tip and tip cone
Foreign particles between the
piston, the O-ring and the cylinder
Insufficient amount of grease on
cylinder and O-ring
O-ring damaged
Incorrect operation
Tip incorrectly attached
Calibration altered: caused
by misuse, for example
Unsuitable calibration.
High viscosity liquids may
require recalibration.
1 0
Solution
Attach firmly
Clean tip cones attach
new tips
Clean and grease O-ring
and cylinder.
Grease accordingly
Change the O-ring
Follow instructions carefully
Attach firmly
Recalibrate according
to instructions
Recalibrate with
the liquids in question.