Storage Recommendations - Agilent InfinityLab Poroshell 120 Guide D'utilisation

Column Care
Column regeneration
Column
Agilent InfinityLab
Poroshell Chiral-T and
Chiral-V
Agilent InfinityLab
Poroshell Chiral-CD and
Chiral-CF

Storage Recommendations

Long-term storage of silica-based, bonded phase columns should be in a
pure organic solvent. If the column has previously been used with a buffered
mobile phase, the buffer should first be removed by purging the column with
20 to 30 column volumes of a 50:50 mixture of methanol or acetonitrile and
water, followed by 20 to 30 column volumes of the pure solvent. Before
storing, end fittings should be tightly capped with end plugs to prevent
packing from drying out. Columns may be safely stored for short periods in
most mobile phases. To protect equipment, is it desirable to remove salts
from the instrument and column by purging the column with the same
mobile phase without the buffer (for example, using 30/70 MeOH/H
remove a 30/70 MeOH/20 mM ammonium formate buffered mobile phase).
Re-equilibration is rapid with the original mobile phase when using this
approach and any danger of corrosion from the salts is eliminated. Refer to
recommended storage solvents in the table below.
Column Storage
Column
InfinityLab Poroshell
Chiral-T and V
InfinityLab Poroshell
Chiral-CD and CF
Regeneration procedure
Flush the column with 50/50, ACN/50 mM ammonium
acetate at lower flow rates for at least 2 hours. Then flush
with pure methanol, and equilibrate with mobile phase.
Flush the column with 50/50, ACN/MeOH at lower flow
rates for at least 2 hours. Then equilibrate with mobile
phase.
Regeneration procedure
Pure MeOH or ethanol is recommended for long term storage.
Pure ethanol is recommended for long term storage.
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