Agilent InfinityLab Poroshell 120 Guide D'utilisation page 5

Column Operating Parameters: pH and Temperature
Column
Agilent InfinityLab
Poroshell Chiral-T
Agilent InfinityLab
Poroshell Chiral-V
Agilent InfinityLab
Poroshell Chiral-CD
Agilent InfinityLab
Poroshell Chiral-CF
Note: All silica-based packings have some solubility in pH >6 aqueous mobile
phases. When using silica-based columns at pH >6, best column lifetime is obtained
at lower temperatures (40 °C max) using low buffer concentrations in the range of
0.01 to 0.02 M. Operating at extreme ends of pH and temperature ranges will have a
significant impact on column lifetime.
Maximum Operating Pressures
Column
Agilent InfinityLab Poroshell Chiral-T
Agilent InfinityLab Poroshell Chiral-V
Agilent InfinityLab Poroshell Chiral-CD 2.7 µm
Agilent InfinityLab Poroshell Chiral-CF 2.7 µm
Mobile Phase Selection and Operating Temperatures
Agilent Chiral columns can be used in any type of organic solvents. However,
when switching to or from a normal phase condition to other mobile phases,
please use ethanol or IPA as a transition solvent. Allow at least 10 column
volumes of conditioning time before injection. Care should be taken to
not exceed the pressure limit of the column when transitioning
mobile phases.
Chiral selector
(covalently bonded
onto Agilent Poroshell
particles)
Teicoplanin
Vancomycin
Hydroxypropylated-β-
cyclodextrin
Derivatized cyclofructan
(CF6)
Particle size
2.7 µm
2.7 µm
ENGLISH
Max
operating Recommended
temperature pH range
45 2.5-7
45 2.5-7
45 3-7
45 3-7
Max pressure
400 bar
(6,000 psi)
400 bar
(6,000 psi)
400 bar
(6,000 psi)
400 bar
(6,000 psi)