Agilent InfinityLab Poroshell 120 Guide D'utilisation page 4

Important safety considerations
• All points of connection in liquid chromatographic systems are potential
sources of leaks. Users should be aware of the toxicity or flammability of
their mobile phases.
• Because of the small particle size, dry column packings are respirable.
Columns should only be opened in a well-ventilated area.
• Please adhere to operating pressure limits noted for each column
(see chart). Exceeding these limits will compromise chromatographic
performance and could be unsafe.
Other operating tips
• While generally not harmful to the column, reverse flow should be
avoided.
• Always use high purity reagents and chromatography grade solvent to
prepare your mobile phase. Degas and filter all mobile phase prior to use.
• Disassembling a column will degrade column performance.
• New columns contain organic solvents. See your Column QC
Performance Report for the solvent composition in your column. Initially,
care should be taken not to pass any mobile phase through the column
that may cause a precipitate to form.
• Agilent chiral columns are compatible with water and all common
organic solvents.
• Columns should not be maintained at elevated pH or elevated
temperature when not in use.
• Avoid use of this column outside of recommended pressure, temperature,
and pH ranges for column phase (see next page). Expect reduced lifetime
when operating outside the recommended ranges.