8.3 Fluorescence
Leica DMI3000 B:
The filter slider (5a.9, S.24) is used to operate
the fluorescence module.
• Pull the filter slider out completely to open the
beam path.
• Push the filter slider into the middle position
(1st detent) to bring the blue filter into the
beam path.
• Insert the filter slider fully in order to block the
beam path (shutter position).
• The fluorescence illumination is controlled by
the rotary knob (5a.10, S.24).
• The filter cubes are swiveled manually into
the beam path by turning the incident light tur-
ret disk.
Leica DMI4000 B and Leica DMI6000 B:
• Use the TL/IL function button to switch to fluo-
rescence FLUO.
• Place a specimen on the stage and select a
suitable objective.
• The current fluorescence filter cube will be
displayed on the LeicaScreen.
• You may protect your specimen from fading by
closing the incident light shutter.
To do so, press the SHUTTER button (87.3) on
the front panel.
The following pictogram will appear on the
LeicaScreen:
Changing the fluorescence filter cube
Fixed function buttons on the front panel:
CUBE 1 to CUBE 6 or Cube CCW
Variable function buttons on the front
panel and SmartMove: CUBE CW or CUBE
CCW
Leica Application Suite (LAS) Software
• Focus the image with the knob on the
SmartMove or the focusing wheel and adjust
the intensity with the INT function buttons.
8. Operation
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