8.2 Contrast Methods
All of the contrast methods of the Leica
DMI4000 B and Leica DMI6000 B can be
selected and controlled via the variable function
buttons and the Leica Application Suite (LAS).
The only exceptions are methods that involve
components requiring manual control (e.g.
systems with manual analyzers). The following
section describes the use of the function
buttons on the stand. For instructions on the use
of the software, please refer to the separate
manual.
Contrast methods for the Leica DMI3000 B are
controlled via the manual condenser, the
manual objective turret, as well as turning
knobs and sliders at the microscope.
8.2.1
Bright Field (TL)
Leica DMI3000 B:
• If necessary, adjust the TL bright field position
at the filter slider.
• Set the condenser to the bright field position.
• Remove all other optical components such as
analyzers, polarizers or IC prisms from the beam
path.
• Insert a transmitted light specimen.
• Select your objective
• Set the brightness at the light potentiometer
• Focus the image with the focus wheels.
Leica DMI4000 B and Leica DMI6000 B:
Note:
If all positions of the filter turret are occupied, filter
cube "A" can be swapped for filter cube "A-TL" us-
ing the Leica Application Suite (LAS). TL contrast
methods are possible with that filter cube.
8. Operation
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