Light Sources; Aperture Diaphragm - Leica DM1000 Mode D'emploi

8. Operation

8.7 Light sources

Transmitted light
Adjust the brightness with the dial (45.1).
The numbers on the dial are not absolute values,
but are intended to enable reproducible
settings.
Note:
The
HI PLAN xx SL*
HI PLAN CY xx SL*
(Synchronized Light) objective lines permit
objectives to be changed without having to
adjust the light intensity in addition.
Fluorescence*
Switch on the lamp at the external power unit.
Caution!
Keep the lamphousing at least 10 cm away
from the wall, curtains, wallpaper, books and
other combustible objects!
Fire Hazard!
Please read the separate documentation for the
supply unit.
40
and

8.8 Aperture Diaphragm

The aperture diaphragm (46.3) determines the
resolution, depth of field and contast of the
microscope image. The best resolution is
obtained when the apertures of the objective
and the condenser are roughly the same.
When the aperture diaphragm is stopped down
to be smaller than the objective aperture,
resolving power is reduced, but the contrast is
enhanced. A noticeable reduction in the
resolving power is observed when the aperture
diaphragm is stopped down to less than 0.6x of
the objective aperture and should be avoided
where possible.
In polarization microscopy, stopping down the
aperture diaphragm generally results in more
intense colors.
The aperture diaphragm is set according to the
viewer's subjective impression of the image, the
scale on the dial is just to allow reproducible
settings and does not represent absolute
aperture values.
Fig. 45
1 Brightness control
1