Oblique Illumination; Polarization - Leica DM1000 Mode D'emploi

9.1.4 Oblique Illumination*

• First adjust transmitted light darkfield
• To obtain a relief-like contrast:
Condenser UCA/P:
Rotate condenser disk slightly out of the DF
position
Condensers CL/PH, CLP/PH, and
APL. ACHR.0.9 (P):
Push DF slide in part way out of the DF
position

9.1.5 Polarization*

• Swing the lambda plate of the lambda plate
compensator out if your microscope is
equipped with it
• Insert a specimen and rotate an appropriate
objective into place
• Bring the image into focus and set the Köhler
illumination (→ p. 28)
• Depending on the equipment, the analyzer
may already have been inserted into the tube
mount during the assembly
Alternative:
If the analyzer mount TL* is used:
Insert the analyzer as far as the clickstop into
the microscope stand. The engraving λ must
be on the underside
When using the Pol intermediate tube*:
Switch on the analyzer
• Push the polarizer with the labelled side
upward into the lower opening
!
Attention!
Always use the polarizer with the labelled
side upward, as otherwise the integrated heat
protection filter is ineffective and the special
polarizer will become useless (discoloring!).
• Bring the polarizer and analyzer into cross po-
sition until they reach maximum darkness
• Remove the object or find an empty area of
the specimen
• Push the analyzer into the stand as far as
the 2nd clickstop or switch on the module
• Remove compensators from the light path if
your microscope is equipped with it
• Rotate the polarizer until you observe the
maximum extinction
eyepiece (fig 48)
• Fix the cross position thus determined with
the clamping screw
Fig. 48
Crossing the polarizers when observing through a focusing
telescope or Bertrand lens, high-aperture Pol objective
a exactly crossed, b not exactly crossed
Pos. a cannot be set if there is strain in the condenser or
objective, Pos. b is adequate for polarization contrast.
a
9. Contrast Methods
position in the
b
45