VWR UHP Mode D'emploi page 7

A B
Fig. 5
• Remove the cap (fig. 1D) using the calibration key
(fig. 5A),
• Set the calibration switch (fig. 1E) in its upper position
(fig. 5A),
• Insert the calibration key into the ejector orifice (fig. 1C)
and place it in the calibration screw of the counter
(fig. 5B),
• Turn the key so as the volume indicated by the counter
is equal to the average calculated volume.
• Remove the calibration key and set the calibration switch
in its lower position (fig. 5C), thus going to the pipetting
mode,
• Place the cap of the ejector pushbutton (fig. 5C).
After having conducted the calibration procedure it is
recommended to record recalibration.
CAUTION: Do not rotate the volume adjustment knob
during calibration (fig. 1B).
6. MAINTENANCE
The pipettor requires periodic maintenance, depending
on the applications performed and intensity of use. The
subassemblies subject to action of aggressive solution
vapors, such as shafts elements, should be regularly
inspected and cleaned.
External surfaces of the handgrip, knob and pushbuttons
may be cleaned with a tissue dipped in isopropyl alcohol.
CAUTION: Use of cleaning solutions not recommended
by the manufacturer may impair the safety of the user
and damage the device.
The pipettor can be sterilized in the autoclave at the tem-
perature of 121°C for 20 minutes. After sterilization, the
pipettor should be dried and cooled down to room tem-
11
C
perature. It is recommended to sterilize the pipettors in
autoclave with initial vacuum and drying cycle.
CAUTION:
- Sterilization in other conditions may cause the damage
of the pipettor,
- Set the volume adjustment knob in its upper (unlocked)
position before sterilization.
Repeatability of obtained results does not change if the
pipettor is operated properly and the autoclaving proce-
dure is conducted appropriately. As there can be a slight
change in the dosing accuracy it is recommended:
- To check calibration of the pipettor after 1st, 3rd and
5th autoclaving procedures, and every 10 autoclaving
cycles during further operation of the pipettor.
7. TROUBLESHOOTING
If you notice an improper pipettor operation, identify the
cause and eliminate the fault. To do this, follow the
instruction in the sequence provided. Replacement of
parts should be required only occasionally, and should
not occur under normal pipettor use.
Problem Cause
The liquid is
dispensed too fast
Droplets of liquid
remain
The tips wettability
in the pipettor tips
has increased due
to extensive use
The pipettor tips
immersion is too
shallow
Air bubbles appear The pipettor tips
in the liquid are incorrectly
aspirated into pressed onto
the tips the pipettor shafts
The tip is damaged
or worn out due to
extensive use
ENGLISH
Instructions
Decrease the
dispensing speed
Replace the tips
with new ones
Immerse the tips
deeper according
to the instruction
Press the tips firmly
Replace the tips
with new ones
12