Preparation For Operation; Pipetting Recommendations - VWR UHP Mode D'emploi

A B
Fig. 2
The range of aspirated volume is displayed on the
counter cap (fig. 1G). The volume is displayed by the
counter and consists of four digits, which should be read
from top to bottom. The digits on the lower drum consti-
tute minimum graduation for the particular model. Value
of the minimum graduation (increment) is presented in
Table 1.
Volume may be changed after setting the knob in its top
position (fig. 2B).
After setting the desired volume, the knob (fig. 1B) should
be locked by pushing it into the lower position (fig. 2C).
Examples of counter indications:
1-10 μl 5-50 μl
1 μl 10 μl 5 μl
Counter indications above the horizontal indicators shown
volume in [μl].
To achieve maximum accuracy, the required volume
should be set from higher volume by reducing indica-
tions of the counter.
Example of correct volume setting:
• If the required volume is lower than
the volume set on the counter it is
necessary to reduce indications of
the counter to the required value.
Before achieving the required value it
is necessary to reduce the speed
5
C
50-300 μl
50 μl 50 μl 300 μl
Fig. 3
with which you rotate the adjustment knob (fig. 1B), so
as not to exceed the value (fig. 3).
• If the required volume is higher than the volume set on
the counter it is necessary to increase indications of the
counter to the value which exceeds the required vol-
ume by rotating the bottom drum by about 1/3 of its cir-
cumference. Then, by slowly rotating the adjustment
knob (fig. 1B), reduce the setting to the required value
(fig. 3).
After setting the required volume, the knob (fig. 1B) should
be moved downward to lock the set volume (fig. 2C).

2.2. PREPARATION FOR OPERATION

Place the tips on the shafts of the pipettor. Holding the
pipette vertically, press it against the tips in the rack box,
until the shafts retreat about 1.5 mm into the multichannel
module. The suspension system of the shafts ensures
even and tight sealing of the tips.
The twisting motion does not have to be performed.
See Table 1 for the appropriate tips designed for the given
pipettor model.
CAUTION:
- It is recommended to use the tips recommended by the
manufacturer, as only those tips will ensure accurate
and repeatable liquid measurements.
- Never aspirate liquid into a pipettor without tips attached.

3. PIPETTING RECOMMENDATIONS

Observing the following recommendations will ensure
accuracy and repeatability of liquid sampling.
• While operating VWR UHP pipettor the volume setting
should be locked - the adjustment knob in its lower
position (fig. 2C).
• Operate the pipettor pushbutton slowly and smoothly
when liquid aspiring and dispensing.
• The depth of tips immersion in the aspirated liquid
should be around 2-4 mm.
• While operating, the pipettor should be held in a vertical
position.
• New tips should be pre-rinsed.
ENGLISH
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