Work Safety Instructions; Technical Parameters; Operation; Setting The Volume - VWR UHP Mode D'emploi

The applied colors:
10 μl pipettors – red,
50 μl, 200 μl and 300 μl pipettors – yellow
Pipettors of volume 300 μl should be used with 300 μl tips.

1.2. WORK SAFETY INSTRUCTIONS

Used symbols:
WARNING!: Risk of injury
CAUTION: Potential damage to the device or pipetting
errors
Long-lasting and faultless working of the pipettor
depends to a large extent on its operation. Read the
pipettor operating instructions carefully and comply with
the principles included.
CAUTION:
• The pipettor is designed to work with tips only. Do not
aspirate liquids without tips attached. Aspirated liquid
should not get into the pipettor because this may dam-
age it.
• Use only the pipettor with the tips attached.
• One time use of a tip guarantees safety and eliminates
possible contamination of the aspirated liquid.
• Keep the pipettor clean; do not clean it with chemically
aggressive substances (e.g. acetone).
• Do not hold the pipettor horizontally if there is liquid in
the tip.
• Only use pipettes in accordance with the manufacturer
instruction to ensure the proper parameters of pipettes.
• In case of improper operation of the pipettor, clean the
pipettor carefully following the instructions given or
send it to the service representative.
WARNING!
• Follow the laboratory work safety regulations.
• Exercise extreme caution while pipetting chemically
aggressive substances. Use the protective equipment,
glasses and gloves.
• Never direct the pipette with the tips attached towards
yourself or anyone else when there is liquid in it.
• Use only the spare parts and accessories recommend-
ed by the manufacturer.
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1.3. TECHNICAL PARAMETERS

VWR UHP (Ultra High-Performance) pipettors are high
quality laboratory instruments, which provide the highest
measurement accuracy and precision.
Accuracy errors and repeatability of liquid volume mea-
surements depend on quality of the applied tips. The
errors provided in the table have been obtained using
VWR tips.
CAUTION: Only those tips guarantee correct compatibili-
ty with the pipettors and ensure accurate and repeatable
liquid aspiration.
Volume Accuracy
Model
[μl] [%]
± 8.0
Min 1
VWR ME-10
± 4.0
5
VWR MT-10
± 2.0
Max 10
± 4.0
Min 5
VWR ME-50
± 3.0
25
VWR MT-50
± 1.6
Max 50
± 3.0
Min 20
VWR ME-200
± 1.5
100
VWR MT-200
± 1.0
Max 200
± 1.6
Min 50
VWR ME-300
± 1.2
150
VWR MT-300
± 1.0
Max 300
Table 1
Accuracy errors and repeatability have been determined
with VWR tips, using a gravimetric method, performing at
least 10 measurements of distilled water at the tempera-
ture 20°±1°C, according to EN ISO 8655 standard.
The pipettor design enables the user to perform calibra-
tion process according to the rules presented in section 5.

2. OPERATION

2.1. SETTING THE VOLUME

Setting the volume is performed by the volume adjust-
ment knob (fig. 1B).
ENGLISH
Precision Increment Fits to tips
[%] [μl] [μl]
≤ 6.0
≤ 2.0
0.01 10
≤ 1.2
≤ 2.5
≤ 1.2
0.05
≤ 0.6
200
≤ 1.5
≤ 0.8
0.2
≤ 0.6
≤ 1.5
≤ 1.0
0.5 300
≤ 0.6
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