Adjusting Transmitted-Light Brightfield On The Full-Köhler Microscope - Zeiss Primo Star Mode D'emploi

Carl Zeiss
• Put the eyepiece into the tube and fix it with the set screw.
• Turn the focusing ring of the eyepiece (Fig. 31/3) to focus on the wedge-shaped figure of the eyepiece
pointer.
• Put the specimen onto the mechanical stage. Look at the specimen through the eyepiece with the
eyepiece pointer and bring the microscopic image into focus using the focusing drive.
• When in the above-mentioned eyepiece both microscopic image and eyepiece pointer appear sharply
defined, focus the image for the second eye by turning the focusing ring of the second eyepiece.
Having done so, both microscopic images inclusive of the eyepiece pointer are focused.
Afterwards, you should focus onto the specimen using the focusing drive only.
Fig. 32 Adjusting transmitted-light
brightfield
• Look through one eyepiece of the binocular tube (Fig. 32/7) and bring the specimen into focus using
the focusing drive (Fig. 32/2).
• Then, readjust image sharpness for the other eye, if necessary, by turning the eye lens of the
adjustable eyepiece.
• Close the luminous-field diaphragm (Fig. 32/3) until it becomes visible (even if not in focus) in the field
of view (Fig. 32/A).
40
Start-Up and Operation
3.6.3
• Rotate the 10x objective into the light path
using the knurled ring (Fig. 32/6) of the
nosepiece.
• First, place a high-contrast specimen slide with
the 0.17 mm cover glass being on top in the
specimen holder of the mechanical stage. Fix
the slide by means of the spring lever
(Fig. 14/19).
• If the microscope stand is equipped with a
phase or dark-field slider, pull this slider out to
the left as far as it will go (phase slider with two
phase contrast positions in mid-position).
• Adjust illumination intensity using the rotary
knob (Fig. 32/1) on the microscope stand.
• Move the Abbe condenser up to the limit stop
using the knurled knob (Fig. 14/22); set the
control lever of the aperture diaphragm
(Fig. 14/21) to mid-position.
The
adjustment of the condenser is on the
left of the microscope if you use the
mechanical stage 75x30 with drive on
the right. It is on the right, if the stage
controls are on the left.
415500-7344-008
Primo Star / Primo Star iLED
Adjusting transmitted-light
brightfield on the Full-Köhler
microscope
knurled knob for vertical
11/2013