9. Put a specimen on the stage and focus.
10. Begin the observation by rotating the DIC slider
knob to ⑧ obtain a three-dimensional sample ef-
fect. (Fig. 86)
11. Insert the desired fluorescence filter and open the
shutter ⑨. (Fig. 87)
12. Adjust the transmitted light intensity to opti-
mize fluorescence and DIC observation un-
til the best image contrast can be achieved.
Page 38
⑧
Fig. 86
⑨
Fig. 87