Pipette Operation; Pipetting Techniques - Thermo Fisher Scientific Finnpipette FOCUS 4600100 Guide D'utilisation

Setting the delivery volume
1. Rapid adjustment: Set the delivery volume using the push
button on the top of the pipette. To increase the delivery
volume, turn the push button counterclockwise.
To decrease the delivery volume, turn it clockwise.
2. Fine adjustment: Set the last digit (0-9) of the delivery
volume by turning the fine adjustment ring.
3. Make sure that the desired delivery volume clicks into place
and that the digits are completely visible in the display window.
The adjustable volume is limited to the specific volume range.
Using excessive force to turn the push button outside the range may jam
the mechanism and eventually damage the pipette.
Tip ejection
To help eliminate the risk of contamination, each pipette is fitted with a tip
ejector system.
The tip ejector system consists of a soft-touch tip ejector and specially
designed gearing mechanism. To release the tip, point the pipette at suitable
waste receptacle and press the tip ejector with your thumb.
Shelf hanger
You can attach the pipette shelf hanger on a counter, pipette stand or
anywhere where you want to hang your pipette.
Clean the area where you plan to attach the shelf hanger. Apply two stickers
to the underside of the shelf hanger. Press the shelf hanger firmly into place
— on a shelf, counter top or pipette stand. To use, hang the grippy finger rest
on the shelf hanger.
General
Special care shall be taken to avoid liquid being aspirated into the interior
working of the pipette.
Push and release the push button slowly at all times, particularly when working
with high viscosity liquids. Never allow the push button to snap back.
Make sure that the tip is firmly attached to the tip cone. Check for foreign particles in the tip.
Before you begin your actual pipetting work, fill and empty the tip 3-5 times with the
solution that you will be pipetting. Hold the pipette in an upright position while aspirating
liquid. The grippy should rest on your index finger. Make sure that the tips, pipette and
solution are at the same temperature.
Forward technique
Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button to the first stop.
2. Dip the tip under the surface of the liquid in
the reservoir to a depth of about 1 cm and
slowly release the push button.
Withdraw the tip from the liquid touching it
against the edge of the reservoir to remove
excess liquid.
3. Deliver the liquid by gently depressing the
push button to the first stop. After a delay of
about one second, continue to depress the
push button all the way to the second stop.
This action will empty the tip.
4. Release the push button to the ready position.
If necessary, change the tip and continue pipetting.

Pipette operation

Pipetting techniques

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