Thermo Fisher Scientific Finnpipette FOCUS 4600100 Guide D'utilisation page 6

Reverse technique
The reverse technique is suitable for dispensing liquids that have a high viscosity or a
tendency to foam easily. The technique is also recommended for dispensing very small volumes.
Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button all the way to the
second stop.
2. Dip the tip under the surface of the liquid in
the reservoir to a depth of about 1 cm and
slowly release the push button.
This action will fill the tip.
Withdraw the tip from the liquid touching it
against the edge of the reservoir to remove
excess liquid.
3. Deliver the preset volume by gently depressing
the push button to the first stop. Hold the push button at the first stop.
Some liquid will remain in the tip and this should not be included in the delivery.
4. The remaining liquid should either be discarded with the tip or pipetted back into
the container.
Repetitive technique
The repetitive technique offers a rapid and simple procedure for repeated delivery of the
same volume. Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button all the way to the
second stop.
2. Dip the tip under the surface of the liquid in
the reservoir to a depth of about 1 cm and
slowly release the push button.
This action will fill the tip.
Withdraw the tip from the liquid touching
against the edge of the reservoir to remove
excess liquid.
3. Deliver the preset volume by gently depressing
the push button to the first stop. Hold the push button at the first stop. Some liquid will
remain in the tip and this should not be included in the delivery.
4. Continue pipetting by repeating steps 2 and 3.
Pipetting of heterogeneous samples
(deproteinization in blood glucose determination, for example)
Use steps 1 and 2 of the forward technique to fill the tip with sample.
Wipe the tip carefully with a dry clean tissue.
1. Immerse the tip into the reagent and depress
the push button to the first stop, making sure
the tip is well below the surface.
2. Release the push button slowly to the ready
position.
This will fill the tip. Keep the tip in the solution.
3. Depress the push button to the first stop and
release slowly.
Keep repeating this procedure until the
interior wall of the tip is clear.
4. Finally, depress the push button all the way to the second stop to completely empty
the tip.
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