Autoclaving; Preparations For Sterile Operation - BRAND seripettor Mode D'emploi

Autoclaving

Both the seripettor
pensing cartridge at 121 °C (250 °F), 2 bar absolute (30 psi) with a holding time of at least 15 minutes
according to DIN EN 285.
1.
Unscrew the pump assembly and dispensing cartridge
(see page 43).
2.
Close the valve block loosely with the closure cap
(Accessories, page 47).
3.
Insert the autoclavable filling tube with the sealing ring at the up-
per end into the filling valve (Accessories, seripettor
page 47).
4.
Loosely screw the closed valve block with the filling tube and
discharge tube onto the filled bottle.
5.
Close the air vent opening with an autoclavable sterile filter
(0.2 µm) (fig. 5).
6.
Open the discharge tube and loosen the locking nut (fig. 6).
7.
Autoclave.
After autoclaving, the instrument should be used only after it has
reached room temperature.
(Exception: seripettor
Note:
It is the user's responsibility to ensure effective autoclaving.

Preparations for sterile operation

1.
After autoclaving, close the discharge tube and tighten
the screw coupling (fig. 1).
2.
Remove the closure cap in a clean bench hood.
3.
Screw in the sterile dispensing cartridge (Accessories, page 45).
4.
Fasten the pump assembly.
44
pro instruments are autoclavable without pump assembly and dis-
and seripettor
® ®
with agar culture media up to 60 °C).
®
5
filling tube,
®
6
1