Thermo Scientific Finnpipette Digital Guide D'utilisation page 8

Test liquid: Water, distilled or deionized, "grade 3" water conforming ISO 3696. Tests are done
in a draft-free room at a constant (±0.5°C) temperature of water, pipette and air between 15°C
to 30°C. The relative humidity must be above 50%. Especially with volumes under 50 µl the
air humidity should be as high as possible to reduce the effect of evaporation loss. Special
accessories, such as the evaporation trap, are recommended.
PROCEDURE TO CHECk CALIBRATION
The pipette is checked with the maximum volume (nominal volume) and with the minimum
volume. A new tip is first pre-wetted 3-5 times and a series of ten pipettings is done with
both volumes. A pipette is always adjusted for delivery (Ex) of the selected volume. Use of
forward pipetting technique is recommended. The maximum permissible errors are designed for
forward method. It is recommended to calibrate all channels of the multichannel pipette, but a
suitable method is also to calibrate only the edge channels. Adjustment check prior calibration is
done using one of the middle channels. It is recommended to use the ISO8655 calibration limits
presented in the following tables or user-defined specifications.
Procedure:
1. Do 10 pipettings with the minimum volume.
2. Do 10 pipettings with the maximum volume.
3. Calculate the inaccuracy (A) and imprecision (cv) of both series.
4. Compare the results to the limits.
If the calculated results are within the selected limits, the adjustment of the pipette is correct.
ISO8655 calibration specifications
Thermo Scientific Finnpipette Digital specifications according to ISO8655 standard.
Finnpipette Digital Single Channel
Range Volume
0,2-2 µl
0,5-10 µl
2-20 µl
5-50 µl
10-100 µl
20-200 µl
100-1000 µl
1-5 ml
2-10 ml
Finnpipette Digital Multichannel
Range Channel
0.5-10 µl 8, 12
5-50 µl 8, 12, 16
50-300 µl 8, 12
Inaccuracy
µl µl
2 ±0.080
0.2 ±0.080
10 ±0.120
1 ±0.120
20 ±0.20
2 ±0.20
50 ±0.50
5 ±0.50
100 ±0.80
10 ±0.80
200 ±1.60
20 ±1.60
1000 ±8.0
100 ±8.0
5000 ±40.0
1000 ±40.0
10000 ±60.0
2000 ±60.0
Volume
µl
10
1
50
5
300
50
8
Imprecision
% s.d. µl
±4 0.040
±40 0.040
±1.2 0.080
±12 0.080
±1.0 0.10
±10.0 0.10
±1.0 0.20
±10 0.20
±0.8 0.30
±8.0 0.30
±0.8 0.60
±8.0 0.60
±0.8 3.0
±8.0 3.0
±0.8 15.0
±4.0 15.0
±0.6 30.0
±3.0 30.0
Inaccuracy
µl %
±0.24 ±2.4
±0.24 ±24
±1.0 ±2.0
±1.0 ±20
±8.0 ±2.7
±8.0 ±16
cv%
2.0
20.0
0.8
8.0
0.5
5.0
0.4
4.0
0.3
3.0
0.3
3.0
0.3
3.0
0.3
1.5
0.3
1.5
Imprecision
s.d.µl cv%
0.16 1.6
0.16 16
0.4 0.8
0.4 8.0
3.0 1.0
3.0 6