3. Remove the specimen from the stage.
4. Turn the polarizer knob Ⓗ under the condenser to achieve
maximum darkening of the eyepieces, and then tighten the
polarizer locking screw Ⓖ. (Fig. 61)
5. Once the maximum darkening is achieved, remove the slider
from the nosepiece, remove the analyzer from the dummy
slider and insert it into the DIC prism. Now insert the DIC
slider ⑥ into the slot ⑤. (Fig. 62)
6. Rotate the condenser turret ⑦ to insert the DIC prism match-
ing the objective in use. (Fig. 63)
•
The condenser is supplied with magnetic markers. Each
marker is specific to the type of insert mounted in the
condenser (DIC, PH, DF, etc.).
7. Put a specimen on the stage and focus.
8. Begin the observation by turning the knob on the DIC slider
⑧ to obtain a three-dimensional effect of the sample. (Fig.
64)
•
Simultaneous observation in DIC (Koehler or Nomarski)
+ fluorescence is not possible.
⑦
Page 32
Ⓗ
Ⓖ
⑥
⑤
F ig. 61
F
ig. 61
F ig. 62
F
ig. 62
F ig. 63
F
ig. 63
⑧
F ig. 64
F
ig. 64