Aperture Diaphragm (As); Transmitted Light Diaphragms (B-510Met-R); Centering The Condenser - Optika Italy B-510 Serie Manuel D'utilisation

9.8.3 Aperture diaphragm (AS)
1. Move the selector ①
illuminator in the position corresponding to letter
"R" (only B-510MET-R). (Fig. 23)
2. Completely pull out the aperture diaphragm
selector ④ to fully close the diaphragm. (Fig. 26)
3. Remove one eyepiece.
4. While looking into the empty eyepiece sleeve,
use the provided Allen wrench into the centering
screws ⑤ to bring the bright spot in the center of
the field of view. (Fig. 26)
5. The illuminator is centered when the diaphragm
image is simmetrical to the field of view.
• The Numerical Aperture (N.A.) value of the
aperture diaphragm affects the image contrast.
Increasing or reducing this value one can vary
resolution, contrast and depth of focus of the
image.
• With low contrast specimens move the diaphragm
lever to about 70%-80% of the objective's N.A. If
necessary, remove on eyepiece and, looking into
empty sleeve, adjust the condenser's ring in order
to obtain an image like the one in Fig. 27.
9.9 Transmitted light diaphragms (B-510MET-R)
9.9.1 Centering the condenser
1. Place the specimen on the stage, insert 10x
objective into the light path and focus.
2. Insert the front lens of the swing-out condenser
①. (Fig. 28)
3. Rotate the field
counterclockwise direction, to fully close the
diaphragm.
4. Rotate the condenser height adjustment knob ③
to focus the edges of the diaphragm.
5. Rotate the two centering screws ④ to bring the
bright spot in the center of the field of view.
6. Gradually open the diaphragm. The condenser
is centered when the diaphragm image is
symmetrical to the field of view.
7. In normal use, open the diaphragm until it
circoscribes the field of view.
on the reflected light
diaphragm ring
②
IRIS DIAPHRAGM
IMAGE
FIELD OF VIEW
③
in
①
②
Page 18
④
⑤
Fig. 26
70-80%
30-20%
Fig. 27
④
Fig. 28