Reverse Pipetting - StarLab ErgoOne 2,5 Mode D'emploi

Reverse pipetting

When pipetting critical viscous media, the precision and accuracy
can be increased through 'reverse pipetting'. In reverse pipetting, a
larger volume is aspirated than that indicated on the volume display.
As a result, a small amount of residual liquid remains in the pipette
tip after the pipetting process. How to reverse pipette:
• Secure the pipette tip.
• Press the volume control button
position.
• Hold the pipette upright and immerse the pipette tip approx. 3mm
into the liquid.
• Allow the volume control button
starting position.
• Hold the pipette tip in the liquid for a further 2 seconds to avoid
sucking in air.
• Remove the pipette tip from the liquid.
• Place the pipette tip against the inner wall of the tube or vessel,
at an angle of 30 – 45°.
• Press the volume control button
reaches the first stop position (do not use the blow-out function).
• Keeping the volume control button
pressed, remove the pipette tip from the tube or vessel before
blowing out the residual liquid into a separate container.
• Allow the volume control button
starting position.
• Press the tip ejector button
Note: Pre-wetting
Some liquids (eg. solutions containing protein, organic solvents,
or viscous liquids) leave a film on the inside of the pipette tip. To
ensure maximum precision and accuracy, STARLAB recommends
pre-wetting each new tip first by aspirating and dispensing the
liquid two or three times. Alternatively, TipOne® RPT pipette tips are
special, surface-treated tips that ensure improved flow behaviour
for 'tricky' liquids.
Ask your STARLAB representative for more information.
8 ErgoOne
until it reaches the second stop
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to return slowly back to the
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slowly and evenly until it
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to return slowly back to the
1
to eject the pipette tip.
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User Manual
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