Calibration; Technical Data; Accuracy Table - BRAND HandyStep touch Mode D'emploi

Possible malfunction
Tip drips
Tip is not recognized

Calibration

Technical data

PD-Tip Volume range
0.1 1 µl ... 100 µl
0.5 5 µl ... 500 µl
1 10 µl ...1 ml
1.25 12.5 µl... 1.25 ml
2.5 25 µl ... 2.5 ml
5 50 µl ... 5 ml
10 100 µl ... 10 ml
12.5 125 µl ... 12.5 ml
25 250 µl ... 25 ml
50 500 µl ... 50 ml
88
Cause
Tip leaky
No coding or coding damaged or tip
not inserted correctly
If the leak test (see „Monitoring volumes" → 87) has been successfully
completed, a gravimetric measurement can determine if the device is within
the limits defined by ISO 8655. The test method required for this is descri-
bed in the testing instructions (SOP).

Accuracy table

The measurements were optained with PD-Tip II by BRAND.
Distilled water was used as the test liquid.
Accuracy A* ≤ ± %
Nominal
Volume increments volume
1 µl ...100 µ 0.1 µl 1
5 µl ... 100 µl 0.1 µl 0.9
100 µl ... 500 µl 1 µl
10 µl ... 1 ml 1 µl 0.6
12.5 µl ... 100 µl 0.5 µl 0.6
100 µl ... 1000 µl 1 µl
1 ml ... 1.25 ml 10 µl
25 µl ... 1000 µl 1 µl 0.5
1 ml ... 2.5 ml 10 µl
50 µl ... 1000 µl 1 µl 0.5
1 ml ... 5 ml 10 µl
100 µl ... 10 ml 10 µl 0.4
125 µl ... 1000 µl 5 µl 0.5
1 ml ...10 ml 10 µl
10 m ...12.5 m 100 µl
250 µl ... 10 ml 10 µl 0.5
10 ml ... 25 ml 100 µl
500 µl ...10 ml 0 µl 0.5
10 ml ... 50 ml 100 µl
The nominal volume is the maximum volume printed on the PD-Tip.
The tolerances specified in ISO 8655 are not exceeded.
Solution
Insert a new tip
Insert a new tip or reinsert, select
the volume size
Coefficient of variation (CV) ≤ %
Nominal
50 % 10 % 1% volume
1 1.6 8 0.5
0.9 1 5 0.3
0.9 1 5 0.3
0.6 0.9 5 0.2
0.6 0.7 3.5 0.15
0.5 0.7 3.5 0.15
0.5 0.7 3.5 0.15
0.5 0.8 3.5 0.15
0.5 0.6 3 0.15
0.5 0.6 3 0.15
50 % 10 % 1 %
1 2 12
0.6 1 5
0.5 0.8 4
0.5 0.7 4
0.3 0.6 3
0.4 0.7 3
0.5 0.8 4
0.6 1.4 6.5
0.3 1.0 6
0.4 1.2 9