Installing Objectives; Substage Condenser; Filters; Operation - Leica DM E Mode D'emploi

The binocular and trinocular bodies maintain a constant tube length
for all interpupillary settings. This means that a change of interpupil-
lary distance does not affect parfocality, magnification or calibrations
which depend on magnification. Interpupillary distance is changed
by simply spreading or pushing eyetubes. They will hold their positi-
on at the appropriate setting.
If you have purchased a standard or custom outfit, you will notice the
objectives are already installed on the nosepiece and the condenser
is in place under the stage. In this case, go to the OPERATION sec-
tion. If you purchased your DM E in components, please continue.

3.2 Installing Objectives

The standard achromatic objectives are safeguarded against theft by
a rotatable exterior plastic sleeve. This plastic sleeve must first be
removed from the actual objective mount before the objective is ins-
erted into the nosepiece. The sleeve is released by pressing in a hole
at the upper edge of the sleeve. This is done with a pointed object,
such as a pen. Attach the objectives to the revolving nosepiece holes
counterclockwise from the lowest magnification to the highest. Always
rotate the nosepiece by using the knurled nosepiece ring.
When the achromatic objectives have been inserted into position,
push the sleeves onto the objectives until the circle on the spring ring
engages in the hole of the sleeve again. Be sure the sleeves have
snapped in place. No special measures are necessary for screwing
other types of objectives into the objective nosepiece.

3.3 Substage Condenser

Adjust the condenser mount to its lowest position, then push the sub-
stage condenser into the fork mount underneath the stage by aligning
the positioning pin into the slot. Two centering screws are included
to properly align the substage condenser. Tighten the two centering
screws to roughly center the substage condenser with the supplied
tool. Each condenser is furnished with an iris diaphragm which con-
trols the effective numerical aperture of the objective. To open and
close this diaphragm, simply rotate the condenser ring to the right or
to the left. Center the condenser in the following way: Close the aper-
ture, raise the condenser to the top, remove an eyepiece and use the
provided tool or set screws with the centering screws to center the
condenser by observing the aperture through the eyetube.

3.4 Filters

The blue filter is factory installed in the underside of the condenser.
For phase contrast applications replace the blue filter with the green
filter. Special care must be used in handling filters. Scratches and fin-
gerprints will result in poor images and lower light levels.
3

4.0 Operation

CAUTION: As a safety precaution, the power cord has been
grounded to the microscope base. Never use an adapter bet-
ween the power cord and the power source; it will render the
grounding feature ineffective.
CAUTION : The top collector lens of the illumination system in
the base of the microscope may become warm or hot. Avoid
touching the lens directly.
4.1 Always use your microscope on a hard, stable surface.
4.2 Plug the microscope power cord into a suitable grounded electri-
cal outlet. A grounded 3-wire cord is provided.
4.3 Turn on the microscope by rotating the illumination control knob
on the bottom left side of the instrument.
4.4 Set the illumination control knob to the lowest setting. The illumi-
nation control allows you to adjust the intensity of light produced
by the illumination system.
4.5 Fully open the aperture diaphragm of the condenser by rotating
the ring to the extreme right.
4.6 Using the substage condenser focusing knob, raise the conden-
ser to the top of its excursion. Critical illumination only: If the con-
denser travel is excessive, limit the travel with the thumb screw
under the substage until the top lens of it is just below the stage
surface (0.35mm).
4.7 Place a specimen slide on the stage.
4.8 Rotate the nosepiece to move the 4x objective into working posi-
tion.
4.9 Raise the stage by rotating the coarse adjustment knob to its posi-
tive stop. Using the fine adjustment knob, bring the specimen into
sharp focus. The DM E has been factory adjusted such that stage
focus can be found 1.5 rotations of focus adjustment.
4.10 Adjust the eyetubes for interpupillary distance and eye differen-
ce.The left eyepiece tube is focusable to compensate for refrac-
tive differences of the eyes. To correctly set the eyetubes, focus
on the specimen through the right eyepiece tube only. Use the fine
adjsutment knob while covering the left eyepiece or closing the
left eye. Next, focus the specimen throught the left eyepiece by
turning the eyetube. Cover the right eyepiece while doing this and
be sure to focus with the left eyetube only, without using the focu-
sing knob.
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