Introduction; The Macsquant Tyto Cell Sorter - Miltenyi Biotec MACSQuant Tyto Cell Sorter Mode D'emploi

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Introduction

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The MACSQuant Tyto Cell Sorter

The MACSQuant Tyto Cell Sorter is an instrument for fluorescence-based cell sorting using a patented microchip
technology with a high-speed mechanical sort valve. The MACSQuant Tyto Cell Sorter measures cell size and cell
complexity, like granularity, with backscatter and side scatter detectors. Backscatter detectors collect the light
that is scattered back from a cell primarily by diffraction of the light around the cell.
The high-frequency fluidic valves embedded in the closed cartridges have a microsecond range of valve
opening and closing cycles. The MACSQuant Tyto Cell Sorter can reach a sort rate up to 30,000 valve actuations
per second (results depend on input sample and Poisson statistics).
Cells pass through the microchip under low positive pressure and no sheath fluid for high cell viability and
functionality, even with fragile cells. The cartridge has aseptic ports for sample administration and collection.
Since the sorting process is not using droplets, no biohazardous aerosols are formed. This minimizes the
operator risk. Samples are also protected from environmental contamination in the closed system of the
MACSQuant Tyto Cell Sorter. Disposable cartridges minimize sample-to-sample carryover and eliminate the
need for sample loading and collection ports. Cells sorted with the MACSQuant Tyto Cell Sorter are ready for use
in sensitive downstream applications such as cell culture and cell expansion.
Figure 1.1: Front view of the MACSQuant Tyto Cell Sorter.
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