Zeiss ApoTome.2 Mode D'emploi page 11

ApoTome.2
9 ESSENTIAL OPERATION CONTROLS
A complete ApoTome.2 imaging system comprises the following components:
microscope
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anti-vibration table or anti-vibration microscope support
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cooled digital camera with high dynamic range
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PC with at least 1 GB RAM according to Carl Zeiss specifications plus monitor, operating system
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Windows ® Vista (32 bit version) or Windows
ApoTome.2 slider with ApoTome control box
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Imaging software ZEN (blue edition)
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This Quick Reference Guide describes the use of the ApoTome.2 hardware. For notes and instructions for
the control (calibration) of the ApoTome.2 through software, please refer to the Operating Manual of the
imaging software ZEN (blue edition).
The control elements of ApoTome.2 are reduced to the two available latch positions in the incident light
beam path and to the operating elements in the imaging software.
The ApoTome.2 hardware consists of two components:
1. slider with transmission grids changer
2. control box
The slider is directly connected to the control box via a cable. The control box is connected to the PC by a
USB cable or, optionally, it can be connected directly to the microscope by a CAN BUS cable. In this case,
the ApoTome.2 directly communicates with the PC via the electronic system of the microscope.
If an Axiovert 200 is used, the control box can only be connected to the PC by a USB cable. A
connection via CAN BUS is not possible!
Major features of the ApoTome.2 include:
− ApoTome.2 slider for the plane of the luminous-field diaphragm in the reflected light path
− ApoTome.2 slider with two click-stop positions:
Click-stop position 1:
An open passage is provided in the reflected light path. Thus, normal fluorescence observations can be
carried out in the wide field, e.g. to find interesting structures and to position the specimens.
Click-stop position 2:
In this position, a glass plate with evaporated grid structure is in the light path. The grid structure is
laterally moved in the specimen plane by means of a scanner mechanism. By capturing three (or more)
images at different grid positions and by subsequent calculation, it is possible to produce an optical
section through the specimen. Three different grid frequencies selected via software are available.
They allow the user to obtain different section thicknesses and to use different objectives.
09/2017
ESSENTIAL OPERATION CONTROLS
® 7 (32 bit version)
423667-6044-008
ZEISS
9