Measuring Liquid
Nitrogen Quantity
Liquid Withdrawal
Filling Instructions
(Thermo 8127)
Thermo Scientific
The procedure below pertains to Thermo 5, 10, 20, and 30 vessels.
1. Use a wooden dipstick. Never use a hollow tube or plastic dipstick to
measure liquid nitrogen.
2. The liquid level will be indicated by a frostline which develops when
the dipstick is removed.
1. Liquid withdrawal for Thermo 5, 10, 20 and 30 is always performed
by pouring or utilizing a withdrawal device (for withdrawal devices, see
Parts List). The withdrawal device pressurizes to approximately 4 PSI;
the pressure forces liquid up the withdrawal tube and out the valve.
2. Always wear proper safety attire.
Warning Liquid nitrogen is extremely cold; it boils at -196°C. Extreme
caution should be exercised when handling cryogenic liquids. Always wear
proper safety attire when transferring liquid nitrogen into or out of
Thermo cylinders. Contact with a cryogenic liquid or cold gas may cause
frostbite to unprotected areas of the body. Protect eyes and skin when
transferring liquid. Stand clear of vent during filling. Loose fitting,
insulated gloves and long sleeves are recommended for arm protection.
Cuffless pants should be worn outside boots or overshoes to shed spilling
liquid. Goggles or face shields should be worn if the possibility of
splashing liquid exists. Failure to observe this warning may lead to severe
burns or eye injuries.
To avoid damage to your dewar, it is important that only the two
following methods be used to fill your vessel. Failure to follow each step
may result in damage which is not covered under the warranty. Read all
instructions carefully. Should questions arise, contact Technical Services.
Section 3
Operation
Cryogenic Transfer Vessels
3-5