Optika Italy IM-3F Manuel D'instructions page 20

Troubleshooting
Review the information in the table below to troubleshoot operating problems.
PROBLEM
I. Optical Section:
The illumination is open, but the field
of view is dark.
The edge of the field of view is
vignetted or the brightness is asym-
metric.
Dust and stains can be seen in the
field of view.
There is an apparent double image.
Poor image quality:
The image is not sharp
The contrast is not high
The details are not clear
The phase contrast is low.
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CAUSE
The plug of the LED holder is
not connected to the illumina-
tion set
The brightness is too low
Too many colour filters have
been stacked
The nosepiece is not in the cor-
rect position
The color filter is partially
inserted
The phase contrast slider is not
in the proper position
There are stains and dust on
the specimen
There are stains and dust on
the eyepiece
The size of the aperture dia-
phragm is too small
The nosepiece is not in the
center of the light path
The aperture diaphragm in
the view of field is opened too
much or too little
The lenses (condenser, objec-
tive, eyepieces are culture
dish) is dirty
In phase contrast observation,
the bottom thickness of the
sample is more than 1.2mm
A bright field objective is used
for phase contrast observation
The condenser ring is not
aligned with the objective
phase ring
The light ring and/or the phase
contrast ring is not centered
The objective used is not com-
patible with the phase ring
The phase contrast depends on
the sample position
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SOLUTION
Connect them
Adjust to a proper setting
Minimize the number of the filters
Turn the nosepiece to a click stop
Insert the filter to full depth
Move the slider to a click stop
Clean the specimen
Clean the eyepiece
Open the aperture diaphragm
Turn the nosepiece to a click stop
Adjust the aperture diaphragm
Thoroughly clean all the optical system
Use a sample holder whose bottom thick-
ness is less than 1.2mm
Switch to a phase contrast objective
Adjust the condenser ring to match the
objective phase ring
Adjust the bolts to center them
Please use a compatible objective
The sample holder is not flat. Move the
sample around until a compatible area is
found.