View field diaphragm
Field diaphragm limits the light beam diameter
on the specimen plane, therefore eliminates
the stray light in order to enhance image con-
trast. When the field diaphragm image is just
at the edge of the field of view, the system can
provide the best performance.
①
Turn the adjusting lever
clock-wise to open the diaphragm, otherwise
to close it. (Fig. 46)
②
Adjust the screws
at both sides of the field
diaphragm to center the image of the dia-
phragm itself. (Fig. 46)
Open the field diaphragm gradually, if the im-
age of field diaphragm is just inscribed to the
field of view, this means that the field dia-
phragm has been centered. (Fig.47)
MICROPHOTOGRAPHY
Installing the photography adapter
1. To activate the video port, pull the light
path selector lever to "In" position. (Fig.48)
2. Loosen the locking bolt ① on the trinocu-
lar viewing tube, and take out the dust cap
②.
3. Install the photography adapter into the
trinocular port according to its instructions,
and screw down the locking bolt ①.
4. Attach the camera ring (if any) to the
adapter.
5. Attach the camera to the ring.
●
Warning: for some cameras (mainly re-
flex) the ring is not included with the mi-
croscope, and it should be supplied by the
user.
●
For the photography of dark specimens,
obscure the eyepieces and the viewfinder
with a dark cloth in order to reduce stray
light.
●
The camera magnification can be calcu-
lated as objective magnification × camera
+ lens magnification.
► When shooting with a SLR, the mirror
movement may cause camera move-
ment. Please lift the mirror, use long
exposure times and use an extension
cord. (Fig.49)
All manuals and user guides at all-guides.com
of field diaphragm
Page 18
②
①
In order to prevent the specimen from fluo-
rescence quenching, don't expose the same
portion of the specimen for too long.
Camera
Adapter
Fig.46
Fig.47
Fig.48
②
①
Fig.49