Dic Observation; Koehler Dic Transmitted Light - Optika Italy B-1000 Serie Manuel D'instructions

11. DIC observation

The microscope allows the observation in Differential Interferential Contrast (DIC) with two different methods:
Koehler DIC and Nomarski DIC.
The Koehler DIC method is the simplest both from the point of view of installation and from the point of view of
use, while the Nomarski DIC method provides for a more complex setup.

11.1 Koehler DIC transmitted light

The Koehler DIC observation in transmitted light re-
quires the kit consisting of the following accessories:
Polarizer ①, transmitted light Analyzer ②, Interferen-
tial green filter ③, DIC slider ④. (Fig. 56)
1. Place the polarizer on the lens at the base of the
microscope.
2. Remove the dummy slider from the nosepiece
and insert the analyzer into the dummy slider,
then insert the ⑤ assembly into the slot ⑥. (Fig.
57)
3. Remove the specimen from the stage.
4. Rotate the polarizer at the base of the microscope
to achieve maximum darkening of the eyepieces.
5. Once the maximum darkening is achieved, re-
move the slider from the nosepiece, remove the
analyzer from the dummy slider and insert it into
the DIC prism. Now insert the DIC slider ⑦ into
the slot ⑥. (Fig. 58)
6. Close the condenser aperture diaphragm a little.
7. Put a specimen on the stage and focus.
8. Begin the observation by rotating the DIC slider
knob ⑧ to obtain a three-dimensional sample ef-
fect. (Fig. 59)
• For a better effect on the image you can use the
green filter IF550 which must be placed above
the polarizer.
③
Page 30
①
②
⑤
⑥
⑦
⑥
⑧
④
Fig. 56
Fig. 57
Fig. 58
Fig. 59