3M Emphaze BV0 3R Instructions D'installation Et D'utilisation page 15

2. Connect the capsule inlet to the reservoir containing the bioprocess fl uid.
3. Start the fl ow of bioprocess fl uid and collect purifi ed permeate.
IMPORTANT NOTICE: For optimal performance, run the fi ltration process at a fl ux of
160 – 630 LMH (0.75-3.0 Bed Volume per minute).
4. Stop the fl ow of bioprocess fl uid at the end of the experiment.
5. The bioprocess fl uid remaining on the upstream side of the capsule may then be processed
through the system:
a. Apply 0.3-0.7 bar (5-10 psig) air or nitrogen gas pressure to vent. Using a non-surging
pump or clean air supply to deliver regulated low-pressure air to push the process fl uid
through the capsule. Do not allow the inlet pressure to pulse or exceed 1.4 bar (20 psig).
b. Monitor the capsule outlet. When liquid fl ow is reduced to a trickle, reduce the system
pressure to 0 bar (0 psig).
3. Capsule Use with Pre-use Autoclave Sterilization
3.A. Recommended Pre-use Autoclave Sterilization of Capsules
1. Wrap the capsule connections and vent with sterilization wrap.
2. Place the capsule in the autoclave.
3. Set-up the autoclave cycle.
a. Select pre-vac cycle for all capsule sizes.
b. Set the sterilization temperature at 121°C.
c. Set the autoclave duration for capsules for 30 minutes.
4. Start the autoclave cycle.
5. At the end of autoclave cycle, remove the capsules from the autoclave and let the capsule
cool down at ambient temperature for at least 60 minutes.
6. Remove the Sterilization Wrap.
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