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6. Align the two ends of the red line with the ruler scale (try to use as many notches as possible to keep the calibration
accuracy).
7. Set the desired unit (micrometer, millimeter or nanometer) in the Calibration dialog box.
8. Insert the length of the drawn line in the Actual Length field (using the same procedure described above).
•
Micrometric slide has two rulers (see chapter 11). One is 1 mm/100 (for biological microscopes) and the other is
10mm/100 (for stereomicroscopes).
•
When using the ruler 1mm/100 the interval between two long lines is 100 mm, while using the ruler 10mm/100 the inter-
val between two long lines is 1000 mm.
9. If everything is fine, click OK to end the calibration. The new magnification (the number you entered in the Magnification
edit box) will be available in the Magnification drop-down list.
10. Repeat the same procedure for each magnification available in your microscope.
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