Pipetting - VITLAB micropipette Mode D'emploi

7. Pipetting

- 5 ml and 10 ml instruments should only be used with the PE
filter installed (see page 30).
- Pipette tips are disposable items!
1. Fitting the tip
Use the correct tips according to the volume range or the color
code.
Ensure that the tips is securely seated.
2. Volume setting
Select the desired volume by rotating the volumes setting wheel.
Avoid twisting and abrupt rotating motions during this adjust-
ment.
3. Aspirate sample
a) Press pipetting button to the first stop.
b) Hold the pipette vertically and immerse the tip 2 to 3 mm into
the liquid.
Volume Immersion
range depth in mm
> 1 µl - 100 µl
> 100 µl - 1000 µl
> 1000 µl
c) Let the pipetting button slide back slowly. In order for the
liquid to reach its end position, leave the tip immersed for
another 1-2 s.
d) Touch the tip against the container wall.
Waiting
time in s
2 - 3 1
2 - 4 1
3 - 6 3
25