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MABTECH INSTRUMENTS
Instrument Decontamination Procedures
The following procedures are examples of instrument decontamination procedures and
serves as support only. Please refer to decontaminant manufacturers' instructions, the
WHO's Laboratory Biosafety Manual or other sources of procedures that may apply at your
company or institute. In case you have any questions, or if you need further advice, please
refer to the person in charge of Environment, Health and Safety issues at your work place,
or to your Mabtech representative.
Biological Agents
The World Health Organisation ("WHO") Laboratory Biosafety Manual describes
decontamination procedures that are used for instruments. Customers are required to refer
to
the
current
https://www.who.int/csr/resources/publications/biosafety/WHO_CDS_CSR_LYO_2004_11
/en/
for identifying the appropriate procedure. The customer must assess the suitability of
these methods for the biological agents concerned and adherence to any warnings in the
instrument's Operating Instructions. Commonly used decontamination agents described in
WHO's manual are:
1. Sodium hypochlorite (1:10 dilution of bleach) that gives 5g/l concentration is a general
all-purpose disinfectant. However, it should be prepared fresh each time. Avoid mixing
bleach with acid as this would release toxic chloride gas.
2. Formaldehyde (Formalin) a solution of gas in water if about 37% concentration. It is
effective for all microorganisms and spores at temperatures > 20°C. Formaldehyde is not
active against prions. It is a suspected carcinogen and safety precautions must be
followed when handling the chemical.
3. Glutaraldehyde is generally supplied in a solution of approximately 2%. It is active
against vegetative bacteria, spores, fungi and lipid/nonlipid viruses. It may take several
hours to kill spores. Glutaraldehyde is toxic and precautions must be taken when
handling the chemical.
4. Phenolic compounds are active against vegetative bacteria and lipid-containing viruses,
and if properly formulated, against mycobacteria. Phenolic compounds are not active
against spores and poorly against non-lipid viruses. Certain phenolic compounds may be
inactivated by water hardness. Phenolic compounds are toxic and can penetrate the skin.
Safety precautions must be followed
5. Alcohols such as 70% ethanol or 70% isopropanol are active against vegetative
bacteria, fungi and lipid-containing viruses but not against spores. Their action against
non-lipid viruses are variable. Alcohols are flammable and must not be used near open
flames or heat.
6. Hydrogen peroxide is a strong oxidant and can be a potent broad-spectrum germicide.
However, a 3-6% solution of hydrogen peroxidase is relatively slow and limited as
version
of
this
manual
(Edition
3)
available
at
3
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