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2. Cleaning the supply and discharge lines
Connect in turn each of the 6 capillary tubings fastened on the connecting piece B to the pump and pump
through ultra pure water.
Check whether solution emerges at the connecting piece B. If one of the supply or discharge lines remained
blocked, the connecting piece B must be replaced (order number 6.2832.010).
3. Reassemble the suppressor and connect it to the IC system
(see Installation, steps 3-5)
Before switching the rotor over for the first time, rinse the three rotor units with solution each for 5 minutes.
Regenerating the suppressor
If the rotor units are loaded with certain heavy metals (e.g. iron) or organic impurities, these can no longer be com-
pletely removed with the regeneration solution normally used. The capacity of the rotor units is consequently affec-
ted, which can result in reduced phosphate sensitivity in less serious cases and a significant rise in the baseline in
more serious cases. In addition, an increased backpressure may be observed in one or several rotor units. If such
capacity or pressure problems occur at one or more positions, all of the 3 rotor units must be regenerated as fol-
lows:
1. Disconnecting the suppressor from the IC system
Disconnect all capillary connections of the connecting piece from the IC system.
2. Regenerating the suppressor
Mount the suppressor on the IC system in such a way that the rotor units can be rinsed in the direction opposite
to the flow of eluents.
To accomplish this, connect the inlet capillary for the regeneration ("regenerant") to the high pressure pump
with the aid of a coupling 6.2744.040 and rinse with one of the solutions suggested below.
Regenerate the 3 rotor units of the MSM-LC, one after the other, with one of the following solutions:
a. In the event of general problems or problems with iron: 60 min. with 0.3 mol/L H
mL/min.
b. In the event of heavy metal contaminations or of elevated backpressure: 15 min. with 1 mol/L H
mol/L oxalic acid
c. In the event of contamination with organic cationic complexing agents: 15 min. with 0.1 mol/L H
mol/L oxalic acid / acetone 5%
d. In the event of severe contamination with organic substances: 15 min. with 0.2 mol/L H
20%
Switch the rotor over to the next position using the STEP command and regenerate it.
In the event of pressure problems, a reduction in pressure should be able to be observed during the regenera-
tion process.
Caution! The 6.1826.xxx pump tubings consist of PVC and therefore must not be used for rinsing with solu-
tions containing acetone.
In this case, use other pump tubings or use a high pressure pump for regenerating.
3. Rinsing the suppressor
After the regeneration has been completed, rinse the rotor units, one after the other, for 15 - 30 min. each, with
degassed ultra pure water (analogous to Step 2).
To accomplish this, dismount the inlet capillary for the regeneration ("regenerant") from the high pressure pump
and connect the inlet capillary for the "rinsing solution" with the high pressure pump.
4. Connecting the suppressor to the IC system
Reconnect the suppressor to the IC system in such a way that conveyance moves in the direction of flow (stand-
ard assembly).
Please note that the rotor first needs to be conditioned prior to beginning measurement.
If the capacity or pressure problems persist, then the rotor must be replaced.
Replacing spare parts
Spare parts may need to be replaced under the following circumstances:
Loss of capacity which cannot be eliminated (reduced phosphate sensitivity and/or significant rise in the base-
￭
line)
Blockage of the rotor which cannot be eliminated (solutions can no longer be pumped through the cartridges of
￭
the rotor)
Defective packing of the rotor (regular large bumps, appearing recurrently in the same form, can be seen on the
￭
chromatogram)
8.110.8008ML
PO
at a flow of 0.6
3
4
SO
+ 0.1
2
4
SO
/ 0.1
2
4
SO
/ acetone ≥
2
4
6/12
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