Optika Italy B-380 Manuel D'utilisation page 10

•
Fully insert the phase contrast condenser into the holder, pushing it until it is well inserted:
•
Rotate the condenser annuli disc to the "BF" position (brightfield).
•
Completely open the aperture diaphragm:
•
Turn on the light and put a sample slide on the stage (an opaque specimen will make alignment easier).
•
Using the 10x objective, center your sample (moving the stage) and focus using the coarse and fine focus
knobs.
•
Rotate the condenser height adjustment knobs until you obtain a uniform white illumination on the sample.
Important: the optimal position corresponds to the top lens of the condenser 1-2mm below the bottom side
of the slide.
•
Rotate the condenser annuli disc to the "10" position.
Condenser must fully enter into its guide
Page 10