Zuzi 4211/20 Y Mode D'emploi page 24

4.2 Quantitative mode
To determine the concentration of different samples from a curve equation in which the absorbance and concen-
tration are related:
C=K·A+B
C: concentration
A: absorbance
K and B: curve coefficients
Select Quantitative with F2 (2.6) to enter the quantitative mode main menu; the following options will be displa-
yed (Fig. 13):
1. Create Curve
2. Load Curve: to load a curve previously created and saved.
3. Remove Curve: to delete a function previously saved.
Figure 13
Create a curve
Select the option Create curve with
be followed will be displayed (Fig. 14):
- Coefficient: this method is used when the equation of the curve is known. First of all, the equation coeffi-
cients must be introduced and then, proceed to the analysis of the samples of unknown concentration.
- Standard curve: this method is used when the equation of the curve is unknown. First of all, the curve
must be created from standard samples of known concentration and then, proceed to the analysis of the samples of
unknown concentration.
Figure 14
1. Coefficient method
Select the option Coefficient with
Figure 15
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100.0% T
(2.7) (2.8) and then confirm OK with F1 (2.5). The two methods that can
(2.7) (2.8) and then confirm OK with F1 (2.5) (Fig. 15).
Instruction manual 542110x0
Work WL.:
Version 2 Nov-10