Bresser MicroSet 40 - 1024x Mode D'emploi page 15

Now
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prepared
(Fig. 1, 19) directly
under the objective on
the microscope stage
I
(Fig. 3). The object
should
directly over the illumination (Fig. 1, 12).
In the next step, take a look through the
eyepiece (Fig. 1, 1) and carefully turn the
focus knob (Fig. 1, 16) until the image
appears clear and sharp.
Now you can select a higher magnifi cation
by slowly removing the Barlow lens (Fig. 1, 3)
from the eyepiece support (Fig. 1, 5). When
the Barlow lens is almost completely pulled
out, the magnifi cation can be increased to
–almost double. If you would like an even
higher level of magnifi cation, insert the 16x
eyepiece (Fig. 1, 2) and turn the objective
nosepiece (Fig. 1, 8) to a higher setting (10x
or 40x).
Important tip:
The highest magnifi cation is not always the
best for every specimen!
Note:
Each time the magnifi cation
(eyepiece or objective change, pulling out the
Barlow lens), the image sharpness must be
place the readjusted with the focus knob (Fig. 1, 16).
specimen When doing this, make sure to be careful. If
you move the microscope stage too quickly,
the objective and the slide could come into
contact and become damaged!
be located For transparent objections (e.g. protozoa), on
the other hand, the light shines from below,
through the opening in the microscope stage
and then through the object.
The light travels further through the objective
and eyepiece, where it is also magnifi ed, and
fi nally goes into the eye. This is transmitted-
light microscopy.
Many microorganisms in water, many plan
components and the smallest animal parts are
already transparent in nature. Others have to
be prepared. We may make them transparent
through a treatment or penetration with
the right materials (media), or by taking the
thinnest slices from them (using our hand or
a specimen slicer), and then examine them.
You can read more about this in the following
sections.
How do I make thin specimen slices?
Only do this with the supervision of your
parents or another adult.
changes As I already pointed out, the thinnest slices
possible are taken from an object. In order to
get the best results, we need some wax or
paraffi n. It is best if you get a candle. Place
the wax in a pot and heat it carefully over a
low burner. Now, dip the object in the liquid
wax a few times. Then, let the wax get hard.
Using the specimen slicer (Fig. 1,19) or a
knife/scalpel, cut the smallest slices from the
object that is covered with wax. These slices
are to be laid on a slide and covered with a
cover slip.
How do I make my own specimens?
Take the object that you want to observe and
place it on a glass slide. Then, add a few
drops of distilled water on the object (Fig.
7) using a pipette (Fig. 1, 19). Now, place a
cover slip vertically at the edge of the drop of
water, so that the water runs along the edge
of the cover slip. Then, slowly lower the cover
slip over the water drops (Fig. 8).
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Note:
The included glue "gum media" (Fig. 1,
18) is used to make permanent prepared
specimens. Use this in place of the distilled
water. If you want to keep the object in place
on the slide permanently, use the gum media.
GB
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